AEP_YARLI_mutated (462 aa)
PSG: a new signal peptide prediction method
N-region: length 2; pos.chg 1; neg.chg 0
H-region: length 23; peak value 0.53
PSG score: -3.88
GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -0.51
possible cleavage site: between 15 and 16
>>> Seems to have no N-terminal signal peptide
ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 2
Number of TMS(s) for threshold 0.5: 1
INTEGRAL Likelihood = -3.66 Transmembrane 3 - 19
PERIPHERAL Likelihood = 5.09 (at 32)
ALOM score: -3.66 (number of TMSs: 1)
MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 10
Charge difference: 0.0 C( 0.0) - N( 0.0)
N >= C: N-terminal side will be inside
>>> membrane topology: type 2 (cytoplasmic tail 1 to 3)
MITDISC: discrimination of mitochondrial targeting seq
R content: 0 Hyd Moment(75): 2.64
Hyd Moment(95): 6.15 G content: 0
D/E content: 1 S/T content: 3
Score: -4.57
Gavel: prediction of cleavage sites for mitochondrial preseq
cleavage site motif not found
NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 7.1%
NLS Score: -0.47
KDEL: ER retention motif in the C-terminus: none
ER Membrane Retention Signals: none
SKL: peroxisomal targeting signal in the C-terminus: none
PTS2: 2nd peroxisomal targeting signal: none
VAC: possible vacuolar targeting motif: none
RNA-binding motif: none
Actinin-type actin-binding motif:
type 1: none
type 2: none
NMYR: N-myristoylation pattern : none
Farnesylation/Geranylgeranylation motif: none
memYQRL: transport motif from cell surface to Golgi: none
Tyrosines in the tail: none
Dileucine motif in the tail: none
checking 63 PROSITE DNA binding motifs: none
checking 71 PROSITE ribosomal protein motifs: none
checking 33 PROSITE prokaryotic dna binding motifs: none
Final Results:
36.0 %: extracellular, including cell wall
12.0 %: endoplasmic reticulum
12.0 %: cytoplasmic
12.0 %: nuclear
12.0 %: plasma membrane
8.0 %: mitochondrial
4.0 %: vesicles of secretory system
4.0 %: vacuolar
>> prediction for AEP_YARLI_mutated is exc