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queryProtein (404 aa)
PSG: a new signal peptide prediction method
N-region: length 7; pos.chg 1; neg.chg 0
H-region: length 19; peak value 0.00
PSG score: -4.40

GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): 1.72
possible cleavage site: between 19 and 20

>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 1
Number of TMS(s) for threshold 0.5: 1
INTEGRAL Likelihood = -3.40 Transmembrane 1 - 17
PERIPHERAL Likelihood = 1.85 (at 156)
ALOM score: -3.40 (number of TMSs: 1)

MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 8
Charge difference: -4.0 C(-2.0) - N( 2.0)
N >= C: N-terminal side will be inside

>>> membrane topology: type 2 (cytoplasmic tail 1 to 1) MITDISC: discrimination of mitochondrial targeting seq
R content: 1 Hyd Moment(75): 5.44
Hyd Moment(95): 8.55 G content: 0
D/E content: 1 S/T content: 6
Score: -2.08

Gavel: prediction of cleavage sites for mitochondrial preseq
R-2 motif at 17 IRL|SL

NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 11.6%
NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals:
KKXX-like motif in the C-terminus: KGSY

SKL: peroxisomal targeting signal in the C-terminus: none

PTS2: 2nd peroxisomal targeting signal: none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
type 1: none
type 2: none

NMYR: N-myristoylation pattern : none

Farnesylation/Geranylgeranylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs: none

checking 71 PROSITE ribosomal protein motifs: none

checking 33 PROSITE prokaryotic dna binding motifs: none