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queryProtein (287 aa)
PSG: a new signal peptide prediction method
N-region: length 7; pos.chg 1; neg.chg 4
H-region: length 6; peak value 0.00
PSG score: -4.40

GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -12.09
possible cleavage site: between 57 and 58

>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 6
INTEGRAL Likelihood = -6.00 Transmembrane 60 - 76
INTEGRAL Likelihood = -1.91 Transmembrane 91 - 107
INTEGRAL Likelihood = -3.08 Transmembrane 134 - 150
INTEGRAL Likelihood = -1.06 Transmembrane 178 - 194
INTEGRAL Likelihood = -5.26 Transmembrane 210 - 226
INTEGRAL Likelihood = -0.64 Transmembrane 257 - 273
PERIPHERAL Likelihood = 4.19 (at 109)
ALOM score: -6.00 (number of TMSs: 6)

MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 67
Charge difference: 2.0 C( 2.0) - N( 0.0)
C > N: C-terminal side will be inside

>>> membrane topology: type 3b MITDISC: discrimination of mitochondrial targeting seq
R content: 0 Hyd Moment(75): 3.36
Hyd Moment(95): 3.85 G content: 1
D/E content: 2 S/T content: 0
Score: -8.17

Gavel: prediction of cleavage sites for mitochondrial preseq
cleavage site motif not found

NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 8.0%
NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals:
KKXX-like motif in the C-terminus: FKSK

SKL: peroxisomal targeting signal in the C-terminus: none

PTS2: 2nd peroxisomal targeting signal: none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
type 1: none
type 2: none

NMYR: N-myristoylation pattern : none

Farnesylation/Geranylgeranylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs: none

checking 71 PROSITE ribosomal protein motifs: none

checking 33 PROSITE prokaryotic dna binding motifs: none