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queryProtein (507 aa)
PSG: a new signal peptide prediction method
N-region: length 2; pos.chg 0; neg.chg 1
H-region: length 20; peak value 0.00
PSG score: -4.40

GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -7.74
possible cleavage site: between 24 and 25

>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 1
Number of TMS(s) for threshold 0.5: 1
INTEGRAL Likelihood = -7.70 Transmembrane 6 - 22
PERIPHERAL Likelihood = 2.76 (at 210)
ALOM score: -7.70 (number of TMSs: 1)

MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 13
Charge difference: 3.5 C( 4.0) - N( 0.5)
C > N: C-terminal side will be inside

>>> membrane topology: type 1b (cytoplasmic tail 6 to 507) MITDISC: discrimination of mitochondrial targeting seq
R content: 2 Hyd Moment(75): 6.62
Hyd Moment(95): 7.58 G content: 4
D/E content: 2 S/T content: 5
Score: -5.37

Gavel: prediction of cleavage sites for mitochondrial preseq
R-2 motif at 73 LRD|LA

NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 12.4%
NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: none

SKL: peroxisomal targeting signal in the C-terminus: none

PTS2: 2nd peroxisomal targeting signal: none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
type 1: none
type 2: none

NMYR: N-myristoylation pattern : none

Farnesylation/Geranylgeranylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: too long tail

Dileucine motif in the tail: found
LL at 21
LL at 107
LL at 205
LL at 274
LL at 278
LL at 373
LL at 374

checking 63 PROSITE DNA binding motifs: none

checking 71 PROSITE ribosomal protein motifs: none

checking 33 PROSITE prokaryotic dna binding motifs: none