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queryProtein (542 aa)
PSG: a new signal peptide prediction method
N-region: length 8; pos.chg 0; neg.chg 2
H-region: length 39; peak value 0.00
PSG score: -4.40

GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -0.23
possible cleavage site: between 39 and 40

>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 10
INTEGRAL Likelihood =-11.46 Transmembrane 27 - 43
INTEGRAL Likelihood =-11.57 Transmembrane 57 - 73
INTEGRAL Likelihood = -6.21 Transmembrane 116 - 132
INTEGRAL Likelihood = -1.54 Transmembrane 212 - 228
INTEGRAL Likelihood = -3.35 Transmembrane 231 - 247
INTEGRAL Likelihood = -4.88 Transmembrane 274 - 290
INTEGRAL Likelihood = -1.86 Transmembrane 309 - 325
INTEGRAL Likelihood = -6.21 Transmembrane 352 - 368
INTEGRAL Likelihood = -0.37 Transmembrane 388 - 404
INTEGRAL Likelihood = -9.61 Transmembrane 422 - 438
PERIPHERAL Likelihood = 0.63 (at 89)
ALOM score: -11.57 (number of TMSs: 10)

MTOP: Prediction of membrane topology (Hartmann et al.)
Center position for calculation: 34
Charge difference: -1.5 C(-2.5) - N(-1.0)
N >= C: N-terminal side will be inside

>>> membrane topology: type 3a MITDISC: discrimination of mitochondrial targeting seq
R content: 0 Hyd Moment(75): 5.29
Hyd Moment(95): 7.48 G content: 0
D/E content: 2 S/T content: 2
Score: -6.40

Gavel: prediction of cleavage sites for mitochondrial preseq
cleavage site motif not found

NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 6.5%
NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: none

SKL: peroxisomal targeting signal in the C-terminus: none

PTS2: 2nd peroxisomal targeting signal: none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
type 1: none
type 2: none

NMYR: N-myristoylation pattern : none

Farnesylation/Geranylgeranylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs:
Leucine zipper pattern (PS00029): *** found ***
LMMLMAYLSYMLAELFNLSGIL at 260
none

checking 71 PROSITE ribosomal protein motifs: none

checking 33 PROSITE prokaryotic dna binding motifs: none